biotinylated secondary antibody (OriGene)
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OriGene
biotinylated secondary antibody
Biotinylated Secondary Antibody, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/biotinylated+secondary+antibody/Biotin+Goat+Polyclonal+Antibody/pm42176116-76-11-14
Average 90 stars, based on 1 article reviews
Biotinylated Secondary Antibody, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/biotinylated+secondary+antibody/Biotin+Goat+Polyclonal+Antibody/pm42176116-76-11-14
Average 90 stars, based on 1 article reviews
biotinylated secondary antibody - by Bioz Stars,
2026-09
90/100 stars
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Incubation:Article Title: Chaperone-mediated autophagy ameliorates hyperlipidemia-induced apoptosis in podocytes via attenuating lipid accumulation. Article Snippet: the past 30 years, global cases of chronic kidney disease (CKD) attributable to type 2 diabetes (T2D) have increased by 74%, while the proportion of ESRD cases caused by diabetes concurrently rose from 22.1% in 2000 to 31.3% in 2015 (Wang et al. 2025a, b).. Diabetes mellitus often accompanied lipid disorders, typified by high circulating levels of triglycerides, free fatty acids (FFAs) and cholesterol, which could cause ectopic lipid accumulation in non-adipose tissues (Rosario and Prabhakar 2006).. Excess accumulation of lipid in non-adipose cells can cause cell dysfunction and cell death, which is termed as lipotoxicity (Sletten et al. 2018). Article Title: Epigenetic silencing of JAM3 promotes laryngeal squamous cell carcinoma development by inhibiting the Hippo pathway Article Snippet: Sections were then blocked with 5% bovine serum albumin (BSA; Beijing Solarbio Science & Technology Co., Ltd.) for 20 min at room temperature to reduce non-specific binding, then incubated overnight at 4°C with primary antibodies against Ki-67 (cat. no. RMA-0731; Fuzhou Maixin Biotechnology Development Co., Ltd.), JAM3 (cat. no. bs-11086R; 1:200; rabbit; BIOSS), YAP1 (cat. no. 13584-1-AP; 1:500; rabbit; Proteintech Group, Inc.), E-Cadherin (cat. no. 20874-1-AP; 1:2,000; rabbit; Proteintech Group, Inc.), N-Cadherin (cat. no. 22018-1-AP; 1:2,000, rabbit; Proteintech Group, Inc.) and Vimentin (cat. no. 5741S; 1:600; rabbit; Cell Signaling Technology, Inc.). .. Following primary antibody incubation, slides were washed with PBS and incubated with a Article Title: sEH inhibition attenuates mtROS-mediated NLRP3 inflammasome activation by promoting mitophagy in tubular epithelial cells in diabetic kidney disease Article Snippet: .. After PBS washes, sections were incubated with Article Title: Synergistic effects of denosumab and BMP-2 on bone regeneration in critical-sized femoral defects in ovariectomized rats. Article Snippet: Next, the sections were incubated overnight at 4°C with primary antibodies against OPG (1:100; Sigma–Aldrich, St. Louis, USA) and OPN (1:200; Biomedical Technologies, Stoughton, MA), using optimized dilutions for best results. .. After three brief washes with PBS, the sections were treated with Article Title: Hypoxia-Induced VGF Promotes Cell Migration and Invasion in Prostate Cancer via the PI3K/Akt Axis Article Snippet: The SP Immunohistochemistry Commercial Detection Kit (SP-9000, Beijing Zhongshan Jinqiao Biotechnology Co., Ltd., Beijing, China) was used to perform immunostaining in accordance with the instructions. .. Following that, they were incubated for 15 min in a working solution of streptavidin/peroxidase complex and then kept for 30 minutes at 37 °C with the kit’s Article Title: AGTR1 potentiates the chemotherapeutic efficacy of cisplatin in esophageal carcinoma through elevation of intracellular Ca 2+ and induction of apoptosis Article Snippet: Next, the slides were incubated with 10% goat serum (cat. no. ZLI-9022; Beijing Zhongshan Jinqiao Biotechnology Co., Ltd.) in PBST (0.1% Tween 20) for 30 min at room temperature to block the unspecific binding of the antibodies. .. After incubation with the primary antibodies against AGTR1 (2.5 μ g/ml dilution; cat. no. 124505; Abcam) or Ki-67 (2.5 μ g/ml; cat. no. ab15580; Abcam) overnight at 4°C, the slides were washed three times in PBST and then incubated with Article Title: Identification and Validation of the Key Genes of Diabetic Vasculopathy: Evidence Based on Bioinformatics Analysis and Animal Study Article Snippet: The section was incubated with primary antibody against BMP4 (Abcam, ab155033; 1/400) at 4°C overnight. .. The section was then incubated using a Staining:Article Title: Chaperone-mediated autophagy ameliorates hyperlipidemia-induced apoptosis in podocytes via attenuating lipid accumulation. Article Snippet: the past 30 years, global cases of chronic kidney disease (CKD) attributable to type 2 diabetes (T2D) have increased by 74%, while the proportion of ESRD cases caused by diabetes concurrently rose from 22.1% in 2000 to 31.3% in 2015 (Wang et al. 2025a, b).. Diabetes mellitus often accompanied lipid disorders, typified by high circulating levels of triglycerides, free fatty acids (FFAs) and cholesterol, which could cause ectopic lipid accumulation in non-adipose tissues (Rosario and Prabhakar 2006).. Excess accumulation of lipid in non-adipose cells can cause cell dysfunction and cell death, which is termed as lipotoxicity (Sletten et al. 2018). Microscopy:Article Title: Chaperone-mediated autophagy ameliorates hyperlipidemia-induced apoptosis in podocytes via attenuating lipid accumulation. Article Snippet: the past 30 years, global cases of chronic kidney disease (CKD) attributable to type 2 diabetes (T2D) have increased by 74%, while the proportion of ESRD cases caused by diabetes concurrently rose from 22.1% in 2000 to 31.3% in 2015 (Wang et al. 2025a, b).. Diabetes mellitus often accompanied lipid disorders, typified by high circulating levels of triglycerides, free fatty acids (FFAs) and cholesterol, which could cause ectopic lipid accumulation in non-adipose tissues (Rosario and Prabhakar 2006).. Excess accumulation of lipid in non-adipose cells can cause cell dysfunction and cell death, which is termed as lipotoxicity (Sletten et al. 2018). Avidin-Biotin Assay:Article Title: Synergistic effects of denosumab and BMP-2 on bone regeneration in critical-sized femoral defects in ovariectomized rats. Article Snippet: Next, the sections were incubated overnight at 4°C with primary antibodies against OPG (1:100; Sigma–Aldrich, St. Louis, USA) and OPN (1:200; Biomedical Technologies, Stoughton, MA), using optimized dilutions for best results. .. After three brief washes with PBS, the sections were treated with |